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相关口腔诊所
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幽门螺杆菌在牙菌斑中的分布研究
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发表时间:2007-1-6 12:27:00
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更多保健方面的文章请到中国保健网>>
摘要 目的:为探讨幽门螺杆菌(hp)在口腔内的分布及感染方式,本实验对不同程度牙周炎患者46例,对照组20例进行了龈上及龈下菌斑研究。方法:采用pcr技术,提取hp的染色体dna,对其特异性片段进行诊断。结果:牙周炎组hp阳性率平均39.1%,健康对照组15%,重症牙周炎组阳性率52.3%,显示了hp在口腔分布与年龄、牙位、牙周炎的程度密切相关。结论:牙菌斑是hp孳生地,口腔环境可能是hp一条传播通道,有关hp对牙周组织损害的研究尚待深入。 关键词 幽门螺杆菌;牙菌斑;牙周炎;pcr 中图号:r780.2 文献标识码:a 文章编号:1005-2593(1999)01-0001-03 [牙体牙髓牙周病学杂志,1999;9(1):1]
study the distribution of helicobacter pylori in dentalplaque by polymerase chain reaction
pan yaping, yie qianhong, wang hongyang
department of periodontology, stomatological college of china medical university, shenyang 110002
abstract aim:whether the oral cavity is a potential reservoir and possible sanctuary for helicobacter pylori, we investigated the distribution of helicobacter pylori and its infection in tooth plaque. methods: forty-six patients with periodontitis and twenty controls with healthy were studied in the supergingival and subgingival plaques by polymerase chain reaction. results: 18 patients(39.1%) with periodontitis was identified in their plaque, controls was 15%; to severe periodontitis,the hp positive rate 52.3%; the distribution of hp had relation with age, sex, teeth site,activities of periodontitis. conclusions: the detection of hp in dental plaques suggested that hp colonization is not restricted to the gastric mucous and this ecological niche may serve be as a possible sanctuary which may be responsible for resettle of the stomach hp. complete treatment to periodontitis is very important to periodontal and stomach health. key words helicobacter pylori; tooth plaque; periodontitis;pcr [chinese journal of conservative dentistry, 1999,9(1):1] 幽门螺杆菌(helicobacter pylori, hp)是一种微需氧、革兰氏阴性螺旋形细菌,与慢性胃炎、胃十二指肠溃疡、胃癌等消化系统疾病的发病关系密切。虽然关于hp的流行病学研究较多,但其传播途径目前仍然不甚了解。国外某些学者近期发现hp也存在于牙菌斑中,提示口腔环境可能是一条hp的传播通道。口腔微生物的种类及数量非常庞大,在深牙周袋及根管内存在大量革兰氏阴性厌氧杆菌,可动菌,但培养及鉴定较为困难。本研究选择克隆的hp染色体dna特异片段,通过获得极少量细菌dna的方法,运用pcr技术研究幽门螺杆菌在口腔菌斑中的分布,探讨hp与牙周病的相互关系,旨在为幽门螺杆菌传播方式及流行病学研究提供有关数据。1 材料和方法1.1 选择对象 实验组:中国医科大学口腔医院牙周科门诊患者46例,均为成人牙周炎(诊断按照全国统编教材第三版口腔内科学标准)。男25例,女21例,平均年龄39.8岁。牙位选择为每一患者仅取一个牙齿的一个牙面菌斑,前牙8个,前磨牙13个,磨牙25个。所有患者在检查前2个月均未服用抗生素,去除龈上菌斑,取龈下菌斑,对于牙龈指数为3以上者,同时留取龈上菌斑,置入装有无菌生理盐水的尖底离心管,-40℃保存备检。 对照组:选择口腔卫生状况良好,无牙周疾病者20例,男11例,女9例,平均年龄35.3岁,前牙3个,前磨牙5个,磨牙12个,与实验组同样取龈下菌斑备检。本新闻共3页,当前在第1页123
责任编辑:姚红祥
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